Identifying the RNA Polymerases that Synthesize Specific Transcripts of the Autographa Californica Nuclear Polyhedrosis Virus
نویسندگان
چکیده
منابع مشابه
Identifying the RNA polymerases that synthesize specific transcripts of the Autographa californica nuclear polyhedrosis virus.
Nuclear run-on assays carried out in the presence and absence of the RNA polymerase II inhibitor, alpha-amanitin, were used to determine the exact timing of the switch from inhibitor-sensitive transcription catalysed by host RNA polymerase II, to inhibitor-resistant transcription catalysed by the baculovirus-induced RNA polymerase. These studies revealed that the onset of alpha-amanitin-resista...
متن کاملTime Course Analysis and Mapping of Autographa californica Nuclear Polyhedrosis Virus Transcripts.
To study the expression of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome, intracellular virus-specific proteins and mRNAs were pulsed-labeled, extracted, and analyzed at 6-h intervals during the replicative cycle. Most RNAs were detected between 12 and 24 h postinfection (p.i.), but many continued to be synthesized until late in infection. Polyhedrin and p8 mRNAs were the...
متن کاملAnalysis of the promoter of the Autographa californica nuclear polyhedrosis virus p10 gene.
Functional analyses of the p10 gene promoter from the Autographa californica nuclear polyhedrosis virus (AcNPV) were performed by progressively deleting the 230 nucleotides upstream from the p10 coding sequences towards the ATG codon. Truncated promoter sequences retaining the full 5' non-coding leader of p10 were inserted in front of the chloramphenicol acetyltransferase (CAT) gene, and promot...
متن کاملExpression of foreign proteins on the surface of Autographa californica nuclear polyhedrosis virus.
Based on the method of direct cloning into the baculovirus genome by linearizing and re-ligation in presence of the target insert, we designed viral constructs that express foreign genes on the surface of baculovirus particles. We chose the glycosylated envelope protein gp41 of human immunodeficiency virus type 1 (HIV-1) as a model for displaying recombinant proteins on budded virus. The ectodo...
متن کاملImproving promiscuous mammalian cell entry by the baculovirus Autographa californica multiple nuclear polyhedrosis virus
The insect baculovirus AcMNPV (Autographa californica multiple nuclear polyhedrosis virus) enters many mammalian cell lines, prompting its application as a general eukaryotic gene delivery agent, but the basis of entry is poorly understood. For adherent mammalian cells, we show that entry is favoured by low pH and by increasing the available cell-surface area through a transient release from th...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1990
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-71-1-195